Batch BLAST Database of NCBI

 
  Upload Query Sequences      
        fasta format zip-directory format
        AA sequence DNA sequence
        Be Sure to Select File and Sequence Types!
 
  Choose Program    Blastn Blastx Blastp tBlastx tBlastn
 
  Choose database      Nucleotide Sequence  Peptide Sequence
 
    

 
  Options   for advanced blasting
 
  Genetic codes   
 
  Choose filter    DUST with blastn, SEG with others Using lower case filter
 
  Expect Value   
 
  Reward      for a nucleotide match
 
  Penalty      for a nucleotide mismatch
 
  GI's    Show GI's in deflines     Restrict search to list of GI's
 
  Word Size    balstn 11, megablast 28, all others 3
 
  Threshold for extending hits    blastn or megablast 0, blastp 11, blastx 12, tblastn or tblastx 13
 
  Matrix   
 
  Search against database     Query strands to search against database( for blast[nx], and tblastx )
 
  Gapped alignment     Ungapped extension   Final alignment 
 
  Cost    to open a gap   Cost to extend a gap
 
  Alignment view